isotype control antibody clone 2a3 Search Results


2a3  (Becton Dickinson)
90
Becton Dickinson 2a3
List of fluorochrome-conjugated monoclonal antibodies used in this study to characterize BMMCs from SM patients and normal/reactive BM.
2a3, supplied by Becton Dickinson, used in various techniques. Bioz Stars score: 90/100, based on 1 PubMed citations. ZERO BIAS - scores, article reviews, protocol conditions and more
https://www.bioz.com/product/isotype+control+antibody+clone+2a3/2a3+antibody/pmc06387409-52-2-6
Average 90 stars, based on 1 article reviews
2a3 - by Bioz Stars, 2026-09
90/100 stars
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h00003556-m03, 3d8; h00003556-m06, 2a3  (Abnova)
90
Abnova h00003556-m03, 3d8; h00003556-m06, 2a3
List of fluorochrome-conjugated monoclonal antibodies used in this study to characterize BMMCs from SM patients and normal/reactive BM.
H00003556 M03, 3d8; H00003556 M06, 2a3, supplied by Abnova, used in various techniques. Bioz Stars score: 90/100, based on 1 PubMed citations. ZERO BIAS - scores, article reviews, protocol conditions and more
https://www.bioz.com/product/isotype+control+antibody+clone+2a3/h00003556+m03++3d8++h00003556+m06++2a3+antibody/pmc05987926-205-8-7
Average 90 stars, based on 1 article reviews
h00003556-m03, 3d8; h00003556-m06, 2a3 - by Bioz Stars, 2026-09
90/100 stars
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cd25 pe-cy7  (Becton Dickinson)
90
Becton Dickinson cd25 pe-cy7
CD4, CD8, regulatory and follicular T cell subsets in bronchoalveolar lavage (BAL), peripheral blood mononuclear cells (PBMC) and lymph nodes (LLN) from sarcoidosis patients.
Cd25 Pe Cy7, supplied by Becton Dickinson, used in various techniques. Bioz Stars score: 90/100, based on 1 PubMed citations. ZERO BIAS - scores, article reviews, protocol conditions and more
https://www.bioz.com/product/isotype+control+antibody+clone+2a3/anti+cd3/pmc09146853-49-31-34
Average 90 stars, based on 1 article reviews
cd25 pe-cy7 - by Bioz Stars, 2026-09
90/100 stars
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anti-cd25 pecy7  (Becton Dickinson)
90
Becton Dickinson anti-cd25 pecy7
Multivariable regression analysis of the effects of age and baseline immune parameters on the magnitude of peak VZV-specific Th1 immune responses to ZVL in the primary cohort ( n = 399) a
Anti Cd25 Pecy7, supplied by Becton Dickinson, used in various techniques. Bioz Stars score: 90/100, based on 1 PubMed citations. ZERO BIAS - scores, article reviews, protocol conditions and more
https://www.bioz.com/product/isotype+control+antibody+clone+2a3/streptavidin+pe/pmc06639287-406-45-52
Average 90 stars, based on 1 article reviews
anti-cd25 pecy7 - by Bioz Stars, 2026-09
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anti cd25  (fluidigm)
94
fluidigm anti cd25

Anti Cd25, supplied by fluidigm, used in various techniques. Bioz Stars score: 94/100, based on 1 PubMed citations. ZERO BIAS - scores, article reviews, protocol conditions and more
https://www.bioz.com/product/isotype+control+antibody+clone+2a3/Anti-Human+CD25+(2A3)-149Sm/pmc11416690-71-0-7
Average 94 stars, based on 1 article reviews
anti cd25 - by Bioz Stars, 2026-09
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anti human pd l1 175  (fluidigm)
93
fluidigm anti human pd l1 175

Anti Human Pd L1 175, supplied by fluidigm, used in various techniques. Bioz Stars score: 93/100, based on 1 PubMed citations. ZERO BIAS - scores, article reviews, protocol conditions and more
https://www.bioz.com/product/isotype+control+antibody+clone+2a3/Anti-Human+CD274%2FPDL1+(29E%2E2A3)-175Lu/leelatian_nalin__2018__stratification_of_adult_glioblastoma_with_signaling_and_single_cell_biology-3084-168-172
Average 93 stars, based on 1 article reviews
anti human pd l1 175 - by Bioz Stars, 2026-09
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anti human pd l1 antibody  (Revvity)
91
Revvity anti human pd l1 antibody
EV71 + IECs express lower levels of MICA/B and higher levels of <t>PD-L1</t> and are less susceptible to iNK and CD3 + iIELs lysis. (A) The intestinal intraepithelial lymphocytes from the C57BL/6 mouse were separated and extracted, and the ratio of CD3 + iIELs and NK1.1 + iNK cells was analyzed by flow cytometry. (B) HT29 cells were infected with EV71 at an MOI of 1 for 48 h. LDH was applied to analyze the susceptibility of EV71 – HT29 and EV71 + HT29 to NK-92 cell lysis at 6 h at the indicated E:T ratios. (C,E) The intestinal intraepithelial lymphocytes were prepared to enrich iNK or CD3 + iIELs using the MojoSort™ Mouse NK Cell Isolation Kit or the MojoSort™ Mouse CD3 T Cell Isolation Kit by MACS. Cells were stained with anti-NK1.1 antibody or anti-CD3 antibody. (D,F) The h-SCARB2-MC38 cells were infected with EV71 at an MOI of 1 for 48 h. LDH was applied to analyze the susceptibility of EV71 – h-SCARB2-MC38 and EV71 + h-SCARB2-MC38 to iNK or CD3 + iIELs cell lysis on 10 h at the indicated E:T ratios. (G,H) HT29 cells were infected with EV71 at an MOI of 1. The membrane expression levels of MICA/B or PD-L1 were detected at 48 hpi by FACS. (I,J) At 48 hpi, cells were fixed and stained with anti-EV71 (green) antibodies, anti-MICA/B or PD-L1 (red) antibodies, and DAPI (blue) and examined by a confocal microscope. Mock was cells without infection. Merged images of the different channels were shown. (K,L) The expression levels of NKG2D or PD-1 on NK-92 were detected by FACS. (M,N) The EV71 – HT29 and EV71 + HT29 were pre-incubated with saturating concentrations of anti-MICA/B Ab (10 μg/ml) or anti-PD-L1 Ab (10 μg/ml), or isotype-matched control antibody at 37°C for 2 h and then washed for use as target cells. The NK-92 cells and treated HT29 were co-incubated for 6 h, and the cytotoxicity of NK-92 cells was detected at an E:T ratio of 5:1 on 6 h by LDH assay. Dates were presented as mean ± SD ( n = 3 independent experiments, * P < 0.05, ** P < 0.01, *** P < 0.001, and *⁣*⁣** P < 0.0001).
Anti Human Pd L1 Antibody, supplied by Revvity, used in various techniques. Bioz Stars score: 91/100, based on 1 PubMed citations. ZERO BIAS - scores, article reviews, protocol conditions and more
https://www.bioz.com/product/isotype+control+antibody+clone+2a3/PD-L1+(human)%2C+lyophilized/pmc08848745-109-23-28
Average 91 stars, based on 1 article reviews
anti human pd l1 antibody - by Bioz Stars, 2026-09
91/100 stars
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anti human cd274 pd l1 29e 2 a3 148nd  (fluidigm)
93
fluidigm anti human cd274 pd l1 29e 2 a3 148nd
EV71 + IECs express lower levels of MICA/B and higher levels of <t>PD-L1</t> and are less susceptible to iNK and CD3 + iIELs lysis. (A) The intestinal intraepithelial lymphocytes from the C57BL/6 mouse were separated and extracted, and the ratio of CD3 + iIELs and NK1.1 + iNK cells was analyzed by flow cytometry. (B) HT29 cells were infected with EV71 at an MOI of 1 for 48 h. LDH was applied to analyze the susceptibility of EV71 – HT29 and EV71 + HT29 to NK-92 cell lysis at 6 h at the indicated E:T ratios. (C,E) The intestinal intraepithelial lymphocytes were prepared to enrich iNK or CD3 + iIELs using the MojoSort™ Mouse NK Cell Isolation Kit or the MojoSort™ Mouse CD3 T Cell Isolation Kit by MACS. Cells were stained with anti-NK1.1 antibody or anti-CD3 antibody. (D,F) The h-SCARB2-MC38 cells were infected with EV71 at an MOI of 1 for 48 h. LDH was applied to analyze the susceptibility of EV71 – h-SCARB2-MC38 and EV71 + h-SCARB2-MC38 to iNK or CD3 + iIELs cell lysis on 10 h at the indicated E:T ratios. (G,H) HT29 cells were infected with EV71 at an MOI of 1. The membrane expression levels of MICA/B or PD-L1 were detected at 48 hpi by FACS. (I,J) At 48 hpi, cells were fixed and stained with anti-EV71 (green) antibodies, anti-MICA/B or PD-L1 (red) antibodies, and DAPI (blue) and examined by a confocal microscope. Mock was cells without infection. Merged images of the different channels were shown. (K,L) The expression levels of NKG2D or PD-1 on NK-92 were detected by FACS. (M,N) The EV71 – HT29 and EV71 + HT29 were pre-incubated with saturating concentrations of anti-MICA/B Ab (10 μg/ml) or anti-PD-L1 Ab (10 μg/ml), or isotype-matched control antibody at 37°C for 2 h and then washed for use as target cells. The NK-92 cells and treated HT29 were co-incubated for 6 h, and the cytotoxicity of NK-92 cells was detected at an E:T ratio of 5:1 on 6 h by LDH assay. Dates were presented as mean ± SD ( n = 3 independent experiments, * P < 0.05, ** P < 0.01, *** P < 0.001, and *⁣*⁣** P < 0.0001).
Anti Human Cd274 Pd L1 29e 2 A3 148nd, supplied by fluidigm, used in various techniques. Bioz Stars score: 93/100, based on 1 PubMed citations. ZERO BIAS - scores, article reviews, protocol conditions and more
https://www.bioz.com/product/isotype+control+antibody+clone+2a3/Anti-Human+CD274%2FPD-L1+(29E%2E2A3)-148Nd/pmc07580234-29-0-4
Average 93 stars, based on 1 article reviews
anti human cd274 pd l1 29e 2 a3 148nd - by Bioz Stars, 2026-09
93/100 stars
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ug mouse isotype control antibody  (Bio X Cell)
95
Bio X Cell ug mouse isotype control antibody
EV71 + IECs express lower levels of MICA/B and higher levels of <t>PD-L1</t> and are less susceptible to iNK and CD3 + iIELs lysis. (A) The intestinal intraepithelial lymphocytes from the C57BL/6 mouse were separated and extracted, and the ratio of CD3 + iIELs and NK1.1 + iNK cells was analyzed by flow cytometry. (B) HT29 cells were infected with EV71 at an MOI of 1 for 48 h. LDH was applied to analyze the susceptibility of EV71 – HT29 and EV71 + HT29 to NK-92 cell lysis at 6 h at the indicated E:T ratios. (C,E) The intestinal intraepithelial lymphocytes were prepared to enrich iNK or CD3 + iIELs using the MojoSort™ Mouse NK Cell Isolation Kit or the MojoSort™ Mouse CD3 T Cell Isolation Kit by MACS. Cells were stained with anti-NK1.1 antibody or anti-CD3 antibody. (D,F) The h-SCARB2-MC38 cells were infected with EV71 at an MOI of 1 for 48 h. LDH was applied to analyze the susceptibility of EV71 – h-SCARB2-MC38 and EV71 + h-SCARB2-MC38 to iNK or CD3 + iIELs cell lysis on 10 h at the indicated E:T ratios. (G,H) HT29 cells were infected with EV71 at an MOI of 1. The membrane expression levels of MICA/B or PD-L1 were detected at 48 hpi by FACS. (I,J) At 48 hpi, cells were fixed and stained with anti-EV71 (green) antibodies, anti-MICA/B or PD-L1 (red) antibodies, and DAPI (blue) and examined by a confocal microscope. Mock was cells without infection. Merged images of the different channels were shown. (K,L) The expression levels of NKG2D or PD-1 on NK-92 were detected by FACS. (M,N) The EV71 – HT29 and EV71 + HT29 were pre-incubated with saturating concentrations of anti-MICA/B Ab (10 μg/ml) or anti-PD-L1 Ab (10 μg/ml), or isotype-matched control antibody at 37°C for 2 h and then washed for use as target cells. The NK-92 cells and treated HT29 were co-incubated for 6 h, and the cytotoxicity of NK-92 cells was detected at an E:T ratio of 5:1 on 6 h by LDH assay. Dates were presented as mean ± SD ( n = 3 independent experiments, * P < 0.05, ** P < 0.01, *** P < 0.001, and *⁣*⁣** P < 0.0001).
Ug Mouse Isotype Control Antibody, supplied by Bio X Cell, used in various techniques. Bioz Stars score: 95/100, based on 1 PubMed citations. ZERO BIAS - scores, article reviews, protocol conditions and more
https://www.bioz.com/product/isotype+control+antibody+clone+2a3/RecombiMAb+mouse+IgG2a+isotype+control%2C+unknown+specificity/pmc07274405-99-37-45
Average 95 stars, based on 1 article reviews
ug mouse isotype control antibody - by Bioz Stars, 2026-09
95/100 stars
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isotype antibodies  (Bio X Cell)
97
Bio X Cell isotype antibodies
EV71 + IECs express lower levels of MICA/B and higher levels of <t>PD-L1</t> and are less susceptible to iNK and CD3 + iIELs lysis. (A) The intestinal intraepithelial lymphocytes from the C57BL/6 mouse were separated and extracted, and the ratio of CD3 + iIELs and NK1.1 + iNK cells was analyzed by flow cytometry. (B) HT29 cells were infected with EV71 at an MOI of 1 for 48 h. LDH was applied to analyze the susceptibility of EV71 – HT29 and EV71 + HT29 to NK-92 cell lysis at 6 h at the indicated E:T ratios. (C,E) The intestinal intraepithelial lymphocytes were prepared to enrich iNK or CD3 + iIELs using the MojoSort™ Mouse NK Cell Isolation Kit or the MojoSort™ Mouse CD3 T Cell Isolation Kit by MACS. Cells were stained with anti-NK1.1 antibody or anti-CD3 antibody. (D,F) The h-SCARB2-MC38 cells were infected with EV71 at an MOI of 1 for 48 h. LDH was applied to analyze the susceptibility of EV71 – h-SCARB2-MC38 and EV71 + h-SCARB2-MC38 to iNK or CD3 + iIELs cell lysis on 10 h at the indicated E:T ratios. (G,H) HT29 cells were infected with EV71 at an MOI of 1. The membrane expression levels of MICA/B or PD-L1 were detected at 48 hpi by FACS. (I,J) At 48 hpi, cells were fixed and stained with anti-EV71 (green) antibodies, anti-MICA/B or PD-L1 (red) antibodies, and DAPI (blue) and examined by a confocal microscope. Mock was cells without infection. Merged images of the different channels were shown. (K,L) The expression levels of NKG2D or PD-1 on NK-92 were detected by FACS. (M,N) The EV71 – HT29 and EV71 + HT29 were pre-incubated with saturating concentrations of anti-MICA/B Ab (10 μg/ml) or anti-PD-L1 Ab (10 μg/ml), or isotype-matched control antibody at 37°C for 2 h and then washed for use as target cells. The NK-92 cells and treated HT29 were co-incubated for 6 h, and the cytotoxicity of NK-92 cells was detected at an E:T ratio of 5:1 on 6 h by LDH assay. Dates were presented as mean ± SD ( n = 3 independent experiments, * P < 0.05, ** P < 0.01, *** P < 0.001, and *⁣*⁣** P < 0.0001).
Isotype Antibodies, supplied by Bio X Cell, used in various techniques. Bioz Stars score: 97/100, based on 1 PubMed citations. ZERO BIAS - scores, article reviews, protocol conditions and more
https://www.bioz.com/product/isotype+control+antibody+clone+2a3/InVivoMAb+rat+IgG2b+isotype+control%2C+anti-keyhole+limpet+hemocyanin/pm26819319-83-5-13
Average 97 stars, based on 1 article reviews
isotype antibodies - by Bioz Stars, 2026-09
97/100 stars
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invivomab anti human pd l1  (Bio X Cell)
95
Bio X Cell invivomab anti human pd l1
EV71 + IECs express lower levels of MICA/B and higher levels of <t>PD-L1</t> and are less susceptible to iNK and CD3 + iIELs lysis. (A) The intestinal intraepithelial lymphocytes from the C57BL/6 mouse were separated and extracted, and the ratio of CD3 + iIELs and NK1.1 + iNK cells was analyzed by flow cytometry. (B) HT29 cells were infected with EV71 at an MOI of 1 for 48 h. LDH was applied to analyze the susceptibility of EV71 – HT29 and EV71 + HT29 to NK-92 cell lysis at 6 h at the indicated E:T ratios. (C,E) The intestinal intraepithelial lymphocytes were prepared to enrich iNK or CD3 + iIELs using the MojoSort™ Mouse NK Cell Isolation Kit or the MojoSort™ Mouse CD3 T Cell Isolation Kit by MACS. Cells were stained with anti-NK1.1 antibody or anti-CD3 antibody. (D,F) The h-SCARB2-MC38 cells were infected with EV71 at an MOI of 1 for 48 h. LDH was applied to analyze the susceptibility of EV71 – h-SCARB2-MC38 and EV71 + h-SCARB2-MC38 to iNK or CD3 + iIELs cell lysis on 10 h at the indicated E:T ratios. (G,H) HT29 cells were infected with EV71 at an MOI of 1. The membrane expression levels of MICA/B or PD-L1 were detected at 48 hpi by FACS. (I,J) At 48 hpi, cells were fixed and stained with anti-EV71 (green) antibodies, anti-MICA/B or PD-L1 (red) antibodies, and DAPI (blue) and examined by a confocal microscope. Mock was cells without infection. Merged images of the different channels were shown. (K,L) The expression levels of NKG2D or PD-1 on NK-92 were detected by FACS. (M,N) The EV71 – HT29 and EV71 + HT29 were pre-incubated with saturating concentrations of anti-MICA/B Ab (10 μg/ml) or anti-PD-L1 Ab (10 μg/ml), or isotype-matched control antibody at 37°C for 2 h and then washed for use as target cells. The NK-92 cells and treated HT29 were co-incubated for 6 h, and the cytotoxicity of NK-92 cells was detected at an E:T ratio of 5:1 on 6 h by LDH assay. Dates were presented as mean ± SD ( n = 3 independent experiments, * P < 0.05, ** P < 0.01, *** P < 0.001, and *⁣*⁣** P < 0.0001).
Invivomab Anti Human Pd L1, supplied by Bio X Cell, used in various techniques. Bioz Stars score: 95/100, based on 1 PubMed citations. ZERO BIAS - scores, article reviews, protocol conditions and more
https://www.bioz.com/product/isotype+control+antibody+clone+2a3/InVivoMAb+anti-human+PD-L1/pmc08718579-48-16-20
Average 95 stars, based on 1 article reviews
invivomab anti human pd l1 - by Bioz Stars, 2026-09
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Image Search Results


List of fluorochrome-conjugated monoclonal antibodies used in this study to characterize BMMCs from SM patients and normal/reactive BM.

Journal: International Journal of Molecular Sciences

Article Title: Bone Marrow Mast Cell Antibody-Targetable Cell Surface Protein Expression Profiles in Systemic Mastocytosis

doi: 10.3390/ijms20030552

Figure Lengend Snippet: List of fluorochrome-conjugated monoclonal antibodies used in this study to characterize BMMCs from SM patients and normal/reactive BM.

Article Snippet: CD25 , 2A3 , PE , BD Biosciences * , Interleukin-2 receptor, subunit α.

Techniques:

CD4, CD8, regulatory and follicular T cell subsets in bronchoalveolar lavage (BAL), peripheral blood mononuclear cells (PBMC) and lymph nodes (LLN) from sarcoidosis patients.

Journal: Life

Article Title: CD103 Expression on Regulatory and Follicular T Cells in Lymph Nodes, Bronchoalveolar Lavage Fluid and Peripheral Blood of Sarcoidosis Patients

doi: 10.3390/life12050762

Figure Lengend Snippet: CD4, CD8, regulatory and follicular T cell subsets in bronchoalveolar lavage (BAL), peripheral blood mononuclear cells (PBMC) and lymph nodes (LLN) from sarcoidosis patients.

Article Snippet: The following mAb were used to detect the different subsets of T and B cells: CD3 APC-Cy7 (OKT3 Biolegend), CD8 BV421 (SK1 Biolegend), CXCR5 PerCPCy5 (J252D4 Biolegend), CD103 PE (mouse IgG1k), CD25 PE-Cy7 (2A3 BD), CD4 FITC (SK3 BD), CD127 APC (hIL-7R-M21 BD) and CD19 PE-Cy7 (SJ25C1).

Techniques:

Multivariable regression analysis of the effects of age and baseline immune parameters on the magnitude of peak VZV-specific Th1 immune responses to ZVL in the primary cohort ( n = 399) a

Journal: Journal of Virology

Article Title: The Effect of Age on the Immunogenicity of the Live Attenuated Zoster Vaccine Is Predicted by Baseline Regulatory T Cells and Varicella-Zoster Virus-Specific T Cell Immunity

doi: 10.1128/JVI.00305-19

Figure Lengend Snippet: Multivariable regression analysis of the effects of age and baseline immune parameters on the magnitude of peak VZV-specific Th1 immune responses to ZVL in the primary cohort ( n = 399) a

Article Snippet: Cells were stained with viability dye (catalog number 423104; BioLegend) and then surface stained with anti-ICOS Ax488 (Alexa Fluor 488) (clone C398.4A, catalog number 313514; BioLegend), anti-CTLA4 PE-CF594 (cyanine-based fluorescent 594) (clone BNI3, catalog number 562742; BD), anti-LAG3 PerCP-Cy5.5 (clone 11C3C65, catalog number 369312; BioLegend), anti-CD25 PECy7 (clone 2A3, catalog number 335807; BD), anti-CD39 APC (clone TU66, catalog number 560239; BD), anti-CD4 Ax700 (clone RPA-T4, catalog number 557922; BD), anti-CD3 APC-H7 (clone SK7, catalog number 560176; BD), and anti-CD127 BV421 (clone A019D5, catalog number 351310; BioLegend).

Techniques:

Multivariable regression analysis of the effects of age and baseline immune parameters on the magnitude of peak VZV-specific CTL and Tfh responses to ZVL in the VZV-specific flow cytometry subset of the primary cohort ( n = 95) a

Journal: Journal of Virology

Article Title: The Effect of Age on the Immunogenicity of the Live Attenuated Zoster Vaccine Is Predicted by Baseline Regulatory T Cells and Varicella-Zoster Virus-Specific T Cell Immunity

doi: 10.1128/JVI.00305-19

Figure Lengend Snippet: Multivariable regression analysis of the effects of age and baseline immune parameters on the magnitude of peak VZV-specific CTL and Tfh responses to ZVL in the VZV-specific flow cytometry subset of the primary cohort ( n = 95) a

Article Snippet: Cells were stained with viability dye (catalog number 423104; BioLegend) and then surface stained with anti-ICOS Ax488 (Alexa Fluor 488) (clone C398.4A, catalog number 313514; BioLegend), anti-CTLA4 PE-CF594 (cyanine-based fluorescent 594) (clone BNI3, catalog number 562742; BD), anti-LAG3 PerCP-Cy5.5 (clone 11C3C65, catalog number 369312; BioLegend), anti-CD25 PECy7 (clone 2A3, catalog number 335807; BD), anti-CD39 APC (clone TU66, catalog number 560239; BD), anti-CD4 Ax700 (clone RPA-T4, catalog number 557922; BD), anti-CD3 APC-H7 (clone SK7, catalog number 560176; BD), and anti-CD127 BV421 (clone A019D5, catalog number 351310; BioLegend).

Techniques: Flow Cytometry

Multivariable regression analysis of the effects of age and baseline immune parameters on the magnitude of persistent VZV-specific Th1 cells 1 year after ZVL administration in the primary cohort ( n = 399) a

Journal: Journal of Virology

Article Title: The Effect of Age on the Immunogenicity of the Live Attenuated Zoster Vaccine Is Predicted by Baseline Regulatory T Cells and Varicella-Zoster Virus-Specific T Cell Immunity

doi: 10.1128/JVI.00305-19

Figure Lengend Snippet: Multivariable regression analysis of the effects of age and baseline immune parameters on the magnitude of persistent VZV-specific Th1 cells 1 year after ZVL administration in the primary cohort ( n = 399) a

Article Snippet: Cells were stained with viability dye (catalog number 423104; BioLegend) and then surface stained with anti-ICOS Ax488 (Alexa Fluor 488) (clone C398.4A, catalog number 313514; BioLegend), anti-CTLA4 PE-CF594 (cyanine-based fluorescent 594) (clone BNI3, catalog number 562742; BD), anti-LAG3 PerCP-Cy5.5 (clone 11C3C65, catalog number 369312; BioLegend), anti-CD25 PECy7 (clone 2A3, catalog number 335807; BD), anti-CD39 APC (clone TU66, catalog number 560239; BD), anti-CD4 Ax700 (clone RPA-T4, catalog number 557922; BD), anti-CD3 APC-H7 (clone SK7, catalog number 560176; BD), and anti-CD127 BV421 (clone A019D5, catalog number 351310; BioLegend).

Techniques:

Multivariable regression analysis of the effects of age and baseline and peak immune parameters on the magnitude of persistent VZV-specific Th1 cells 1 year after ZVL administration in the primary cohort ( n = 399) a

Journal: Journal of Virology

Article Title: The Effect of Age on the Immunogenicity of the Live Attenuated Zoster Vaccine Is Predicted by Baseline Regulatory T Cells and Varicella-Zoster Virus-Specific T Cell Immunity

doi: 10.1128/JVI.00305-19

Figure Lengend Snippet: Multivariable regression analysis of the effects of age and baseline and peak immune parameters on the magnitude of persistent VZV-specific Th1 cells 1 year after ZVL administration in the primary cohort ( n = 399) a

Article Snippet: Cells were stained with viability dye (catalog number 423104; BioLegend) and then surface stained with anti-ICOS Ax488 (Alexa Fluor 488) (clone C398.4A, catalog number 313514; BioLegend), anti-CTLA4 PE-CF594 (cyanine-based fluorescent 594) (clone BNI3, catalog number 562742; BD), anti-LAG3 PerCP-Cy5.5 (clone 11C3C65, catalog number 369312; BioLegend), anti-CD25 PECy7 (clone 2A3, catalog number 335807; BD), anti-CD39 APC (clone TU66, catalog number 560239; BD), anti-CD4 Ax700 (clone RPA-T4, catalog number 557922; BD), anti-CD3 APC-H7 (clone SK7, catalog number 560176; BD), and anti-CD127 BV421 (clone A019D5, catalog number 351310; BioLegend).

Techniques:

Expression of FOXP3 and inhibitory ligands on CD4+ CD25+ CD127− Treg. Data were derived from 6 adults of all ages. The parent population is indicated on the abscissa. Horizontal bars indicate paired comparisons and P values.

Journal: Journal of Virology

Article Title: The Effect of Age on the Immunogenicity of the Live Attenuated Zoster Vaccine Is Predicted by Baseline Regulatory T Cells and Varicella-Zoster Virus-Specific T Cell Immunity

doi: 10.1128/JVI.00305-19

Figure Lengend Snippet: Expression of FOXP3 and inhibitory ligands on CD4+ CD25+ CD127− Treg. Data were derived from 6 adults of all ages. The parent population is indicated on the abscissa. Horizontal bars indicate paired comparisons and P values.

Article Snippet: Cells were stained with viability dye (catalog number 423104; BioLegend) and then surface stained with anti-ICOS Ax488 (Alexa Fluor 488) (clone C398.4A, catalog number 313514; BioLegend), anti-CTLA4 PE-CF594 (cyanine-based fluorescent 594) (clone BNI3, catalog number 562742; BD), anti-LAG3 PerCP-Cy5.5 (clone 11C3C65, catalog number 369312; BioLegend), anti-CD25 PECy7 (clone 2A3, catalog number 335807; BD), anti-CD39 APC (clone TU66, catalog number 560239; BD), anti-CD4 Ax700 (clone RPA-T4, catalog number 557922; BD), anti-CD3 APC-H7 (clone SK7, catalog number 560176; BD), and anti-CD127 BV421 (clone A019D5, catalog number 351310; BioLegend).

Techniques: Expressing, Derivative Assay

Journal: iScience

Article Title: Defects in NK cell immunity of pediatric cancer patients revealed by deep immune profiling

doi: 10.1016/j.isci.2024.110837

Figure Lengend Snippet:

Article Snippet: Anti-CD25 (clone 2A3, conjugated to 149Sm) , Standard BioTools , Cat#3149010B.

Techniques: Purification, Clinical Proteomics, Recombinant, Blocking Assay, Staining, Saline, Mass Cytometry, Software, Cytometry

EV71 + IECs express lower levels of MICA/B and higher levels of PD-L1 and are less susceptible to iNK and CD3 + iIELs lysis. (A) The intestinal intraepithelial lymphocytes from the C57BL/6 mouse were separated and extracted, and the ratio of CD3 + iIELs and NK1.1 + iNK cells was analyzed by flow cytometry. (B) HT29 cells were infected with EV71 at an MOI of 1 for 48 h. LDH was applied to analyze the susceptibility of EV71 – HT29 and EV71 + HT29 to NK-92 cell lysis at 6 h at the indicated E:T ratios. (C,E) The intestinal intraepithelial lymphocytes were prepared to enrich iNK or CD3 + iIELs using the MojoSort™ Mouse NK Cell Isolation Kit or the MojoSort™ Mouse CD3 T Cell Isolation Kit by MACS. Cells were stained with anti-NK1.1 antibody or anti-CD3 antibody. (D,F) The h-SCARB2-MC38 cells were infected with EV71 at an MOI of 1 for 48 h. LDH was applied to analyze the susceptibility of EV71 – h-SCARB2-MC38 and EV71 + h-SCARB2-MC38 to iNK or CD3 + iIELs cell lysis on 10 h at the indicated E:T ratios. (G,H) HT29 cells were infected with EV71 at an MOI of 1. The membrane expression levels of MICA/B or PD-L1 were detected at 48 hpi by FACS. (I,J) At 48 hpi, cells were fixed and stained with anti-EV71 (green) antibodies, anti-MICA/B or PD-L1 (red) antibodies, and DAPI (blue) and examined by a confocal microscope. Mock was cells without infection. Merged images of the different channels were shown. (K,L) The expression levels of NKG2D or PD-1 on NK-92 were detected by FACS. (M,N) The EV71 – HT29 and EV71 + HT29 were pre-incubated with saturating concentrations of anti-MICA/B Ab (10 μg/ml) or anti-PD-L1 Ab (10 μg/ml), or isotype-matched control antibody at 37°C for 2 h and then washed for use as target cells. The NK-92 cells and treated HT29 were co-incubated for 6 h, and the cytotoxicity of NK-92 cells was detected at an E:T ratio of 5:1 on 6 h by LDH assay. Dates were presented as mean ± SD ( n = 3 independent experiments, * P < 0.05, ** P < 0.01, *** P < 0.001, and *⁣*⁣** P < 0.0001).

Journal: Frontiers in Microbiology

Article Title: Enterovirus 71 Antagonizes Antiviral Effects of Type III Interferon and Evades the Clearance of Intestinal Intraepithelial Lymphocytes

doi: 10.3389/fmicb.2021.806084

Figure Lengend Snippet: EV71 + IECs express lower levels of MICA/B and higher levels of PD-L1 and are less susceptible to iNK and CD3 + iIELs lysis. (A) The intestinal intraepithelial lymphocytes from the C57BL/6 mouse were separated and extracted, and the ratio of CD3 + iIELs and NK1.1 + iNK cells was analyzed by flow cytometry. (B) HT29 cells were infected with EV71 at an MOI of 1 for 48 h. LDH was applied to analyze the susceptibility of EV71 – HT29 and EV71 + HT29 to NK-92 cell lysis at 6 h at the indicated E:T ratios. (C,E) The intestinal intraepithelial lymphocytes were prepared to enrich iNK or CD3 + iIELs using the MojoSort™ Mouse NK Cell Isolation Kit or the MojoSort™ Mouse CD3 T Cell Isolation Kit by MACS. Cells were stained with anti-NK1.1 antibody or anti-CD3 antibody. (D,F) The h-SCARB2-MC38 cells were infected with EV71 at an MOI of 1 for 48 h. LDH was applied to analyze the susceptibility of EV71 – h-SCARB2-MC38 and EV71 + h-SCARB2-MC38 to iNK or CD3 + iIELs cell lysis on 10 h at the indicated E:T ratios. (G,H) HT29 cells were infected with EV71 at an MOI of 1. The membrane expression levels of MICA/B or PD-L1 were detected at 48 hpi by FACS. (I,J) At 48 hpi, cells were fixed and stained with anti-EV71 (green) antibodies, anti-MICA/B or PD-L1 (red) antibodies, and DAPI (blue) and examined by a confocal microscope. Mock was cells without infection. Merged images of the different channels were shown. (K,L) The expression levels of NKG2D or PD-1 on NK-92 were detected by FACS. (M,N) The EV71 – HT29 and EV71 + HT29 were pre-incubated with saturating concentrations of anti-MICA/B Ab (10 μg/ml) or anti-PD-L1 Ab (10 μg/ml), or isotype-matched control antibody at 37°C for 2 h and then washed for use as target cells. The NK-92 cells and treated HT29 were co-incubated for 6 h, and the cytotoxicity of NK-92 cells was detected at an E:T ratio of 5:1 on 6 h by LDH assay. Dates were presented as mean ± SD ( n = 3 independent experiments, * P < 0.05, ** P < 0.01, *** P < 0.001, and *⁣*⁣** P < 0.0001).

Article Snippet: For blocking experiments, HT29 infected with or without EV71 were pre-incubated with saturating concentrations of anti-human MICA/MICB antibody (10 μg/ml; Biolegend; Clone, 6D4), anti-human PD-L1 antibody (10 μg/ml; Biolegend; Clone, 29E.2A3), or isotype-matched control antibody at 37°C for 2 h and then washed for use as effector cells.

Techniques: Lysis, Flow Cytometry, Infection, Cell Isolation, Staining, Membrane, Expressing, Microscopy, Incubation, Control, Lactate Dehydrogenase Assay

The 2Apro and 3Cpro reduce the susceptibility of IECs to NK lysis by suppressing NKG2DL expression and improving PD-L1 expression on IECs. (A) Analyze the susceptibility of HT29 cells transfected with the pcDNA3.1-2A (pcDNA3.1) or p-EGFP-3C (p-EGFP) plasmids to NK-92 cell lysis on 6 h at the indicated E:T ratios by LDH. (B,D) HT29 cells were transfected with the four kinds of plasmids. After 24 h transfection, the cells were collected to detect the expression levels of PD-L1 and MICA/B by FACS. (C,E,F) The mRNA expression levels of PD-L1, MICA/B, and ULBP1∼6 were determined by qRT-PCR. Control was cells without any treatment. Dates were presented as mean ± SD ( n = 3 independent experiments, * P < 0.05, ** P < 0.01, *** P < 0.001, *⁣*⁣** P < 0.0001 and ns, not significant).

Journal: Frontiers in Microbiology

Article Title: Enterovirus 71 Antagonizes Antiviral Effects of Type III Interferon and Evades the Clearance of Intestinal Intraepithelial Lymphocytes

doi: 10.3389/fmicb.2021.806084

Figure Lengend Snippet: The 2Apro and 3Cpro reduce the susceptibility of IECs to NK lysis by suppressing NKG2DL expression and improving PD-L1 expression on IECs. (A) Analyze the susceptibility of HT29 cells transfected with the pcDNA3.1-2A (pcDNA3.1) or p-EGFP-3C (p-EGFP) plasmids to NK-92 cell lysis on 6 h at the indicated E:T ratios by LDH. (B,D) HT29 cells were transfected with the four kinds of plasmids. After 24 h transfection, the cells were collected to detect the expression levels of PD-L1 and MICA/B by FACS. (C,E,F) The mRNA expression levels of PD-L1, MICA/B, and ULBP1∼6 were determined by qRT-PCR. Control was cells without any treatment. Dates were presented as mean ± SD ( n = 3 independent experiments, * P < 0.05, ** P < 0.01, *** P < 0.001, *⁣*⁣** P < 0.0001 and ns, not significant).

Article Snippet: For blocking experiments, HT29 infected with or without EV71 were pre-incubated with saturating concentrations of anti-human MICA/MICB antibody (10 μg/ml; Biolegend; Clone, 6D4), anti-human PD-L1 antibody (10 μg/ml; Biolegend; Clone, 29E.2A3), or isotype-matched control antibody at 37°C for 2 h and then washed for use as effector cells.

Techniques: Lysis, Expressing, Transfection, Quantitative RT-PCR, Control

The 2Apro and 3Cpro of enterovirus 71 inhibit the IFN-λ production and IFN-λ receptor expression and further decrease the response of IECs to IFN-λ. Also, 2Apro and 3Cpro reduce the susceptibility of IECs to iNK and CD3 + iIELs lysis, possibly by down-regulation of NKG2DL and up-regulation of PD-L1.

Journal: Frontiers in Microbiology

Article Title: Enterovirus 71 Antagonizes Antiviral Effects of Type III Interferon and Evades the Clearance of Intestinal Intraepithelial Lymphocytes

doi: 10.3389/fmicb.2021.806084

Figure Lengend Snippet: The 2Apro and 3Cpro of enterovirus 71 inhibit the IFN-λ production and IFN-λ receptor expression and further decrease the response of IECs to IFN-λ. Also, 2Apro and 3Cpro reduce the susceptibility of IECs to iNK and CD3 + iIELs lysis, possibly by down-regulation of NKG2DL and up-regulation of PD-L1.

Article Snippet: For blocking experiments, HT29 infected with or without EV71 were pre-incubated with saturating concentrations of anti-human MICA/MICB antibody (10 μg/ml; Biolegend; Clone, 6D4), anti-human PD-L1 antibody (10 μg/ml; Biolegend; Clone, 29E.2A3), or isotype-matched control antibody at 37°C for 2 h and then washed for use as effector cells.

Techniques: Expressing, Lysis